SUMMARY OF PROFESSIONAL EXPERTISE
Plant disease diagnosis
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Observation: Experience in diagnosing plant diseases
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Isolations and preservation: plant, forest, food pathogens, epiphytes, and endophytes. Long & short-term storage and preservation of microorganisms.
Endophytes
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Species diversity and richness analysis
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Biocontrol activity of endophytes in the lab., greenhouse, and field assay
Microscopy
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Microscopic observation of fungi and bacteria, cell, and spore characters
Molecular techniques
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Fungi and bacteria-DNA extraction, PCR, and sequencing
Phylogeny
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Phydit Program, MP–ML analysis Clustal X, Clustal W
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BioEdit program, MEGA program, Phylogenetic tree construction
Plant virus
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Detection, partial & total RNA extraction
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cDNA synthesis, Multiplex PCR (RT–PCR), Electrophoresis & data analysis by LabChip GX Touch HT analyzer
Biocontrol activity
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Antagonists’ selection and activity test, biopesticide preparation, and experimentation
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Antagonistic compound extraction, purification, and identification [TLC, Sephadex LH-20 column chromatography]
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Growth condition optimization [Fungi & bacteria–pH, rpm, time course, media, natural and artificial nutrient]
Plant growth promotion
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Plant growth-promoting fungi (PGPF) & rhizobacteria (PGPR) activity test in the lab. and in planta
Fungicide sensitivity assay
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In vitro fungicide sensitivity assay using different pathogens against different fungicides, including MBC, SDHI, Qo1, and DMI
Breeding and resistance variety selection
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Resistant variety selection against fungal and viral sweet potato diseases
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Method development and improvements for resistant variety selection